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sequence-specific stem-loop rt primers taqman microrna reverse transcription kit  (Thermo Fisher)


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    Thermo Fisher sequence-specific stem-loop rt primers taqman microrna reverse transcription kit
    Sequence Specific Stem Loop Rt Primers Taqman Microrna Reverse Transcription Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stem-loop+reverse+transcription+primers/sequence+specific+stem+loop+rt+primers+taqman+microrna+reverse+transcription+kit/pm40412229-94-1-12
    Average 90 stars, based on 1 article reviews
    sequence-specific stem-loop rt primers taqman microrna reverse transcription kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Role of LCN2 in a murine model of hindlimb ischemia and in peripheral artery disease patients, and its potential regulation by miR-138-5P.
    Article Snippet: For miRNAs, 5 ng of total RNA was retro-transcribed with stem-loop reverse transcription primers (Thermo Fisher Scientific) and MultiScribe reverse transcriptase enzyme (Thermo Fisher Scientific). qPCRs were performed on a ViiA 7 Real-Time PCR System (Thermo Fisher Scientific) using PrimeTime qPCR gene expression assays (IDT) for the protein-coding transcripts and TaqMan® MicroRNA Assays (Thermo Fisher Scientific) for the miRNAs (Supplemental Table 1). β-actin and GAPDH were used as housekeeping genes for mRNA analysis in mouse and human samples respectively.

    Article Title: Reducing In-Stent Restenosis
    Article Snippet: Data were analyzed using Data Assist analysis software, version 3 (Applied Biosystems, CA, USA). miRNA quantification by real-time polymerase chain reaction (qRT-PCR) cDNA was synthesized from RNA using stem-loop reverse transcription primers (applied biosystems, Foster City, CA, USA). qRTPCR was performed using TaqMan® universal master mixII with Taqman microRNA expression probes according to the manufacturer’s instructions.

    Article Title: Dairy Cows Naturally Infected with Bovine Leukemia Virus Exhibit Abnormal B- and T-Cell Phenotypes after Primary and Secondary Exposures to Keyhole Limpet Hemocyanin.
    Article Snippet: BLV miRNA B4-3p, B5-5p, B3-3p, B1-3p, and B2-5p expression was measured using stem-loop reverse transcription primers (Thermo Fisher Scientific) and Taqman microRNA assays (Thermo Fisher Scientific).

    Article Title: Micrornas that silence tau expression
    Article Snippet: 100 ng of total RNA was reverse-transcribed using specific stem-loop reverse transcription primers (Applied Biosystems) and miR-219-5p levels were measured with TaqMan microRNA assays on a Mastercycler ep realplex (Eppendorf, Hamburg, Germany).

    Article Title: microRNA-425-5p is upregulated in human gastric cancer and contributes to invasion and metastasis in vitro and in vivo
    Article Snippet: Briefly, 10 ng total RNA was reverse transcribed to cDNA with specific stem-loop reverse transcription primers (Applied Biosystems Life Technologies).

    Real-time Polymerase Chain Reaction:

    Article Title: Role of LCN2 in a murine model of hindlimb ischemia and in peripheral artery disease patients, and its potential regulation by miR-138-5P.
    Article Snippet: For miRNAs, 5 ng of total RNA was retro-transcribed with stem-loop reverse transcription primers (Thermo Fisher Scientific) and MultiScribe reverse transcriptase enzyme (Thermo Fisher Scientific). qPCRs were performed on a ViiA 7 Real-Time PCR System (Thermo Fisher Scientific) using PrimeTime qPCR gene expression assays (IDT) for the protein-coding transcripts and TaqMan® MicroRNA Assays (Thermo Fisher Scientific) for the miRNAs (Supplemental Table 1). β-actin and GAPDH were used as housekeeping genes for mRNA analysis in mouse and human samples respectively.

    Article Title: Reducing In-Stent Restenosis
    Article Snippet: Data were analyzed using Data Assist analysis software, version 3 (Applied Biosystems, CA, USA). miRNA quantification by real-time polymerase chain reaction (qRT-PCR) cDNA was synthesized from RNA using stem-loop reverse transcription primers (applied biosystems, Foster City, CA, USA). qRTPCR was performed using TaqMan® universal master mixII with Taqman microRNA expression probes according to the manufacturer’s instructions.

    Article Title: Dairy Cows Naturally Infected with Bovine Leukemia Virus Exhibit Abnormal B- and T-Cell Phenotypes after Primary and Secondary Exposures to Keyhole Limpet Hemocyanin.
    Article Snippet: BLV miRNA B4-3p, B5-5p, B3-3p, B1-3p, and B2-5p expression was measured using stem-loop reverse transcription primers (Thermo Fisher Scientific) and Taqman microRNA assays (Thermo Fisher Scientific).

    Article Title: Micrornas that silence tau expression
    Article Snippet: 100 ng of total RNA was reverse-transcribed using specific stem-loop reverse transcription primers (Applied Biosystems) and miR-219-5p levels were measured with TaqMan microRNA assays on a Mastercycler ep realplex (Eppendorf, Hamburg, Germany).

    Article Title: microRNA-425-5p is upregulated in human gastric cancer and contributes to invasion and metastasis in vitro and in vivo
    Article Snippet: Briefly, 10 ng total RNA was reverse transcribed to cDNA with specific stem-loop reverse transcription primers (Applied Biosystems Life Technologies).

    Gene Expression:

    Article Title: Role of LCN2 in a murine model of hindlimb ischemia and in peripheral artery disease patients, and its potential regulation by miR-138-5P.
    Article Snippet: For miRNAs, 5 ng of total RNA was retro-transcribed with stem-loop reverse transcription primers (Thermo Fisher Scientific) and MultiScribe reverse transcriptase enzyme (Thermo Fisher Scientific). qPCRs were performed on a ViiA 7 Real-Time PCR System (Thermo Fisher Scientific) using PrimeTime qPCR gene expression assays (IDT) for the protein-coding transcripts and TaqMan® MicroRNA Assays (Thermo Fisher Scientific) for the miRNAs (Supplemental Table 1). β-actin and GAPDH were used as housekeeping genes for mRNA analysis in mouse and human samples respectively.

    Article Title: Reducing In-Stent Restenosis
    Article Snippet: Data were analyzed using Data Assist analysis software, version 3 (Applied Biosystems, CA, USA). miRNA quantification by real-time polymerase chain reaction (qRT-PCR) cDNA was synthesized from RNA using stem-loop reverse transcription primers (applied biosystems, Foster City, CA, USA). qRTPCR was performed using TaqMan® universal master mixII with Taqman microRNA expression probes according to the manufacturer’s instructions.

    Article Title: Dairy Cows Naturally Infected with Bovine Leukemia Virus Exhibit Abnormal B- and T-Cell Phenotypes after Primary and Secondary Exposures to Keyhole Limpet Hemocyanin.
    Article Snippet: BLV miRNA B4-3p, B5-5p, B3-3p, B1-3p, and B2-5p expression was measured using stem-loop reverse transcription primers (Thermo Fisher Scientific) and Taqman microRNA assays (Thermo Fisher Scientific).

    Article Title: Micrornas that silence tau expression
    Article Snippet: 100 ng of total RNA was reverse-transcribed using specific stem-loop reverse transcription primers (Applied Biosystems) and miR-219-5p levels were measured with TaqMan microRNA assays on a Mastercycler ep realplex (Eppendorf, Hamburg, Germany).

    Article Title: microRNA-425-5p is upregulated in human gastric cancer and contributes to invasion and metastasis in vitro and in vivo
    Article Snippet: Briefly, 10 ng total RNA was reverse transcribed to cDNA with specific stem-loop reverse transcription primers (Applied Biosystems Life Technologies).

    Expressing:

    Article Title: Role of LCN2 in a murine model of hindlimb ischemia and in peripheral artery disease patients, and its potential regulation by miR-138-5P.
    Article Snippet: For miRNAs, 5 ng of total RNA was retro-transcribed with stem-loop reverse transcription primers (Thermo Fisher Scientific) and MultiScribe reverse transcriptase enzyme (Thermo Fisher Scientific). qPCRs were performed on a ViiA 7 Real-Time PCR System (Thermo Fisher Scientific) using PrimeTime qPCR gene expression assays (IDT) for the protein-coding transcripts and TaqMan® MicroRNA Assays (Thermo Fisher Scientific) for the miRNAs (Supplemental Table 1). β-actin and GAPDH were used as housekeeping genes for mRNA analysis in mouse and human samples respectively.

    Article Title: Reducing In-Stent Restenosis
    Article Snippet: Data were analyzed using Data Assist analysis software, version 3 (Applied Biosystems, CA, USA). miRNA quantification by real-time polymerase chain reaction (qRT-PCR) cDNA was synthesized from RNA using stem-loop reverse transcription primers (applied biosystems, Foster City, CA, USA). qRTPCR was performed using TaqMan® universal master mixII with Taqman microRNA expression probes according to the manufacturer’s instructions.

    Article Title: Dairy Cows Naturally Infected with Bovine Leukemia Virus Exhibit Abnormal B- and T-Cell Phenotypes after Primary and Secondary Exposures to Keyhole Limpet Hemocyanin.
    Article Snippet: BLV miRNA B4-3p, B5-5p, B3-3p, B1-3p, and B2-5p expression was measured using stem-loop reverse transcription primers (Thermo Fisher Scientific) and Taqman microRNA assays (Thermo Fisher Scientific).

    Article Title: Micrornas that silence tau expression
    Article Snippet: 100 ng of total RNA was reverse-transcribed using specific stem-loop reverse transcription primers (Applied Biosystems) and miR-219-5p levels were measured with TaqMan microRNA assays on a Mastercycler ep realplex (Eppendorf, Hamburg, Germany).

    Article Title: microRNA-425-5p is upregulated in human gastric cancer and contributes to invasion and metastasis in vitro and in vivo
    Article Snippet: Briefly, 10 ng total RNA was reverse transcribed to cDNA with specific stem-loop reverse transcription primers (Applied Biosystems Life Technologies).

    Software:

    Article Title: Role of LCN2 in a murine model of hindlimb ischemia and in peripheral artery disease patients, and its potential regulation by miR-138-5P.
    Article Snippet: For miRNAs, 5 ng of total RNA was retro-transcribed with stem-loop reverse transcription primers (Thermo Fisher Scientific) and MultiScribe reverse transcriptase enzyme (Thermo Fisher Scientific). qPCRs were performed on a ViiA 7 Real-Time PCR System (Thermo Fisher Scientific) using PrimeTime qPCR gene expression assays (IDT) for the protein-coding transcripts and TaqMan® MicroRNA Assays (Thermo Fisher Scientific) for the miRNAs (Supplemental Table 1). β-actin and GAPDH were used as housekeeping genes for mRNA analysis in mouse and human samples respectively.

    Article Title: Reducing In-Stent Restenosis
    Article Snippet: Data were analyzed using Data Assist analysis software, version 3 (Applied Biosystems, CA, USA). miRNA quantification by real-time polymerase chain reaction (qRT-PCR) cDNA was synthesized from RNA using stem-loop reverse transcription primers (applied biosystems, Foster City, CA, USA). qRTPCR was performed using TaqMan® universal master mixII with Taqman microRNA expression probes according to the manufacturer’s instructions.

    Article Title: Dairy Cows Naturally Infected with Bovine Leukemia Virus Exhibit Abnormal B- and T-Cell Phenotypes after Primary and Secondary Exposures to Keyhole Limpet Hemocyanin.
    Article Snippet: BLV miRNA B4-3p, B5-5p, B3-3p, B1-3p, and B2-5p expression was measured using stem-loop reverse transcription primers (Thermo Fisher Scientific) and Taqman microRNA assays (Thermo Fisher Scientific).

    Article Title: Micrornas that silence tau expression
    Article Snippet: 100 ng of total RNA was reverse-transcribed using specific stem-loop reverse transcription primers (Applied Biosystems) and miR-219-5p levels were measured with TaqMan microRNA assays on a Mastercycler ep realplex (Eppendorf, Hamburg, Germany).

    Article Title: microRNA-425-5p is upregulated in human gastric cancer and contributes to invasion and metastasis in vitro and in vivo
    Article Snippet: Briefly, 10 ng total RNA was reverse transcribed to cDNA with specific stem-loop reverse transcription primers (Applied Biosystems Life Technologies).

    Synthesized:

    Article Title: Role of LCN2 in a murine model of hindlimb ischemia and in peripheral artery disease patients, and its potential regulation by miR-138-5P.
    Article Snippet: For miRNAs, 5 ng of total RNA was retro-transcribed with stem-loop reverse transcription primers (Thermo Fisher Scientific) and MultiScribe reverse transcriptase enzyme (Thermo Fisher Scientific). qPCRs were performed on a ViiA 7 Real-Time PCR System (Thermo Fisher Scientific) using PrimeTime qPCR gene expression assays (IDT) for the protein-coding transcripts and TaqMan® MicroRNA Assays (Thermo Fisher Scientific) for the miRNAs (Supplemental Table 1). β-actin and GAPDH were used as housekeeping genes for mRNA analysis in mouse and human samples respectively.

    Article Title: Reducing In-Stent Restenosis
    Article Snippet: Data were analyzed using Data Assist analysis software, version 3 (Applied Biosystems, CA, USA). miRNA quantification by real-time polymerase chain reaction (qRT-PCR) cDNA was synthesized from RNA using stem-loop reverse transcription primers (applied biosystems, Foster City, CA, USA). qRTPCR was performed using TaqMan® universal master mixII with Taqman microRNA expression probes according to the manufacturer’s instructions.

    Article Title: Dairy Cows Naturally Infected with Bovine Leukemia Virus Exhibit Abnormal B- and T-Cell Phenotypes after Primary and Secondary Exposures to Keyhole Limpet Hemocyanin.
    Article Snippet: BLV miRNA B4-3p, B5-5p, B3-3p, B1-3p, and B2-5p expression was measured using stem-loop reverse transcription primers (Thermo Fisher Scientific) and Taqman microRNA assays (Thermo Fisher Scientific).

    Article Title: Micrornas that silence tau expression
    Article Snippet: 100 ng of total RNA was reverse-transcribed using specific stem-loop reverse transcription primers (Applied Biosystems) and miR-219-5p levels were measured with TaqMan microRNA assays on a Mastercycler ep realplex (Eppendorf, Hamburg, Germany).

    Article Title: microRNA-425-5p is upregulated in human gastric cancer and contributes to invasion and metastasis in vitro and in vivo
    Article Snippet: Briefly, 10 ng total RNA was reverse transcribed to cDNA with specific stem-loop reverse transcription primers (Applied Biosystems Life Technologies).



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